Footage from Bow Wow's (real name Shad Moss) show was posted on Saturday, July 20.
He appeared to have thrown shade at Ciara's husband Russell Wilson when making the crude remark.
"I had this b**ch first," Bow Wow, 32, said on stage. He dated Ciara, 33, long before she linked up with her NFL beau.
Bow Wow's remark didn't sit well with some of his hip-hop peers. "Damn, Cuzz, just damn!" T.I. wrote in the comments section.
Bow Wow and Ciara's relationship was brought up recently by his other ex Erica Mena.
Erica, 31, spoke about up after his former flame Kiyomi Leslie went off on social media about their Super Bowl weekend incident that occurred in February 2019.
The Love & Hip-Hop star claimed that Bow Wow broke Ciara's finger "years ago" and how this issue "been ongoing."